FORSCHUNGSLEITUNG : Univ. Doz. Dr. HANS-JÜRGEN BUSSE....................
DOKTORANDEN :
Mag. Sandra Buczolits
DIPLOMANDEN : Elke Hauser, Hanna Worliczek
In our group the major topic is the isolation of bacteria from various environmental sources and their taxonomic characterization. For classification and identification we are using a set of methods including analyses of 16S rRNA gene sequences, quinone systems, polar lipids, cell wall diamino acids, and polyamine patterns as well as genomic fingerprint techniques (ERIC-, BOX-, REP-, RAPD- PCR and ARDRA) and examination of physiological characteristics. In recent years we have contributed to the description of new taxa of four major lines of descent of the domain Bacteria namely Proteobacteria (Thermomonas, Vibrio, Psychrobacter, Pigmentiphaga, Xenophilus, Sphingomonas, Sphingopyxis, Novosphingobium, Erythrobacter, Ochrobactrum) Actinobacteria (Citricoccus, Agrococcus, Microbacterium, Georgenia, Frigoribacterium, Nocardiopsis) Gram-positives with low G+C content (Bacillus, Virgibacillus), CFB-group (Hymenobacter, Chryseobacterium) and the archaeal taxon Halococcus dombrowskii.
At present we are dealing with the characterization of bacteria which were isolated from livers of laboratory mice and which were obtained during studies on the hygiene status of the Medical Clinic for Ungulates and Small Animals at the University of Veterinary Medicine Vienna.
Further interests are:
Colonization of the stomachs of dogs by Helicobacter and characterization of Helicobacter diversity.
Establishment of polar lipid profiles for classification of Mycoplasma species and related taxa.
Examination of the hygiene status (airborne micro-organisms, surfaces),
PCR-diagnostics of Helicobacter from all sources (other taxa, please request),
Classification and identification of bacteria. from clinical and non-clinical origin.
Neighbour-joining tree showing the distribution of selected specied for which we contributed to their original description. The tree was calculated using the Phylogeny Inference Package (PHYLIP, Felsenstein, 1993; Dep. Genetics, Univ. of Washington, Seattle, WA, USA)
REFERENCES (since 2000)
Wieser, M. and Busse, H.-J. (2000). Rapid identification of Staphylococcus epidermidis. Int. J. Syst. Evol. Microbiol. 50, 1087 - 1093.
Busse, H.-J., Kainz, A., Tsitko, I. and Salkinja-Salonen, M. (2000). Riboprints as a tool for preliminary identification of sphingomonads. System. Appl. Microbiol. 23, 115-123.
Berzobohaty, P., Klose, V., Täubel, M., Busse, H.-J., Lubitz, W. and Baatz, W. (2000). Bakterien auf Wandmalereien - Ein Projektbericht (P9099 MOB des FWF). Resauratorenblätter 20, 24-25.
Kainz, A., Lubitz, W. and Busse, H.-J. (2000). Genomic fingerprints, ARDRA-profiles and quinone systems of Pasteurella sensu stricto. Syst. Appl. Microbiol., 23, 494-503.
Schroll, G., Parrer, G., Busse, H.-J., Rölleke, S., Lubitz, W. and Denner, E. M. B. (2001). Alcaligenes faecalis susp.parafaecalis subsp.nov, a bacterium accumulating poly(ß-hydoxybutyrate) (PHB) on AB (acetone-butanol) bioprocess residues. Syst. Appl. Microbiol., 24, 37-43.
Denner, E. B. M., Mark, B., Busse, H.-J., Turkiewicz, M. and W. Lubitz. (2001). Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the antarctic krill Euphausia superba dana, excreting a cold-adapted metalloprotease. Syst. Appl. Microbiol., 24, 44-53.
Denner, E. B. M., Paukner, S., Kämpfer, P., Moore, E. R. B., Abraham, W.-R., Busse, H.- J., Wanner, G, and Lubitz, W. (2001). Sphingomonas pituitosa sp. nov., an exopolysaccharide-producing bacterium that secretes an unusual type of sphingan. Int. J.
Syst. Evol. Microbiol., 51, 827- 841.
Blümel, S., Busse, H.-J., Stolz A. and Kämpfer, P. (2001). Xenophilus azovorans gen. nov. sp. nov., a soil bacterium that is able to degrade azo dyes of the Orange II type. Int. J. Syst. Evol. Microbiol., 51, 1831-1837.
Blümel, S., Mark, B., Busse, H.-J., Kämpfer, P. and Stolz, A.. (2001). Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically. Int. J. Syst. Evol. Microbiol., 51, 1867-71.
Peltola, J. S. P., Andersson, M. A., Kämpfer, P., Auling, G., Kroppenstedt, R. M., Busse, H-J., Salkinoja-Salonen M. S. and Rainey, F. A. (2001). Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species N. exhalans and N. umidischolae. Appl. Environ. Microbiol., 67, 4293-4304.
Camuffo, D., Van Grieken, R., Busse, H.-J., Sturaro, G., Valentino, A., Bernardi, A., Blades, N., Shooter, D., Gysels, K., Deutsch, F., Wieser, M., Kim, O., Ulrych, U. (2001). Environmental monitoring in four European museums. Atmosph. Environ. 35 Suppl. No 1, S127-S140.
Buczolits, S., Rosengarten, R., Hirt, R. and Busse, H.-J (2001). Classification of a Brevundimonas strain detectable after PCR with a Helicobacter-specific primer pair. Syst. Appl. Microbiol. 24, 368-376
Busse, H.-J., Kämpfer, P., Moore, E. R. B., Nuutinen, J. Tsitko, I. V., Denner, E. B. M., Vauterin, L., Valens, M., Rosselló-Mora, R. and Salkinoja-Salonen, M. S. (2002) Thermomonas haemolytica gen. nov., sp. nov., a new genus of the g-Proteobacteria. Int. J. Syst. Evol. Microbiol. 52, 473–483.
Buczolits, S., Denner, E. B. M., Vybiral, D., Kämpfer, P., Wieser, M. and Busse, H.-J. (2002). Proposal of Hymenobacter aerophilus sp. nov. for two airborne bacterial isolates. Int. J. Syst. Evol. Microbiol. 52, 445–456.
Wieser, M., Denner, E.B.M.,
Kämpfer, P., Schumann, P., Tindall, B., Steiner,U., Vybiral, D., Lubitz, W.,
Maszenan, A. M,. Patel, B. K. C., Seviour, R. J., Radax, C. and Busse, H.-J.
(2002) Emended descriptions of the genus Micrococcus, Micrococcus
luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int.
J. Syst. Evol. Microbiol. 52, 629–637.
Kämpfer, P., Busse, H.-J. and Rainey, F. A. (2002) Nocardiopsis compostus sp. nov., a new Nocardiopsis species from the surrounding air of a composting facility. Int. J. Syst. Evol. Microbiol. 52, 621–627
Denner, E. B. M., Vybiral, D., Fischer, U. R. Velimirov, B. and Busse, H.-J. (2002) Vibrio calviensis sp. nov., a halophilic, facultatively oligotrophic 0.2 µm filterable marine bacterium. Int. J. Syst. Evol. Microbiol. 52, 549–553.
Altenburger, P., Kämpfer, P., Schumann, P., Vybiral, D., Lubitz, W. and Busse, H.-J. (2002) Georgenia muralis gen. nov., sp. nov. a novel actinobacterium isolated from a medieval wall painting. Int. J. Syst. Evol. Microbiol., 52, 875-881.
Kämpfer, P., Neef, A., Salkinoja-Salonen, M. S. and Busse, H.-J. (2002). Chelatobacter heintzii (Auling et al. 1993) is a later subjective synonym of Aminobacter aminovorans (Urakami et al. 1992) . Int. J. Syst. Evol. Microbiol. 52: 835-839.
Zlamala, C., Schumann, P., Kämpfer, P., Rossello-Mora, R., Lubitz, W. and Busse, H.-J. (2002). Agrococcus baldri sp. nov., a novel species isolated from the air in the “Virgilkapelle” in Vienna. Int. J. Syst. Evol. Microbiol. 52, 1211-1216.
Zlamala, C., Schumann, P., Kämpfer, P., Valens, M., Rossello-Mora, R., Lubitz, W. and Busse, H.-J. (2002). Microbacterium aerolatum sp. nov., a novel species isolated from the air in the “Virgilkapelle” in Vienna. Int. J. Syst. Evol. Microbiol., 52, 1229-1234..
Heyrman, J, Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. and De Vos, P. 2003. Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int. J. Syst. Evol. Microbiol., 53, 501-511.
Sturaro G., Camuffo D., Brimblecombe P., Van Grieken R., Busse H.-J., Bernardi A., Valentino A., Blades N., Gysels K., Deutsch, F., Wieser, M. and Buczolitz, S. 2003. Multidisciplinary environmental monitoring at the Kunsthistorisches Museum, Vienna. J. Trace Microprobe Tech. 21, 273-294.
Täubel, M., Kämpfer, P., Buczolits, S., Lubitz, W. and Busse, H.-J. Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int. J. Syst. Evol. Microbiol., 53, 725-730.
Kämpfer, P., Buczolits, S., Albrecht, A., Busse, H.-J. & Stackebrandt, E. Towards a standardised format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int. J. Syst. Evol. Microbiol. 53, 893-896.
Buczolits, S., Hirt, R., Rosengarten, R., und Busse, H.-J. (2003) PCR-based genetic evidence for occurrence of Helicobacter pylori and novel Helicobacter species in the canine gastric mucosa. Vet. Microbiol., in press.
Denner, E. B. M., Smith, G., Busse, H.-J., Schumann, P., Narzt, T., Lubitz, W., and Richardson, L. L. (2003). Characterisation of the bacterial pathogen WP1, the causative agent of plaque type II disease on Caribbean scleractinian corals: description of Aurantimonas coralicida gen. nov., sp. nov. Int. J. Syst. Evol Microbiol. 53, Papers in Press, http://dx.doi.org/10.1099/ijs.0.02359-0.